RNA sequencing in a nutshell - Perspectives and applications in marine biology


Nguyen, Tuan

Research Assistant
Faculty of Science, Health, Education and Engineering
University of the Sunshine Coast
Queensland, Australia

Hurwood, David

Senior Lecturer in Ecology (Molecular Ec)
Science and Engineering Faculty
Queensland University of Technology
Brisbane, Australia

Rotllant, Guiomar

Departament de Recursos Marins Renovables
Institut de Ciències del Mar - CSIC
Barcelona, Spain


The advancement of next generation sequencing has moved biology science toward the post-genomics era. This course will provide the participants with the initial foundation for simple data analysis methodologies in RNA sequencing (RNA-Seq). Lectures will give insight into how biological knowledge can be generated from RNA-Seq and also hands-on training on the latest analytical approaches. This course's target audience is researchers and early stage PhD students in the field of marine biology and aquaculture with little to no knowledge of RNA-Seq technology but planning to explore (and apply) high throughput sequencing technologies and bioinformatics methods in their research.

Programme information: 

Day 1 : Introduction to bioinformatics
09:00 – 9:30 Welcome to the course – Outline of the course / Ice breaker activity
09:30 – 11:00 Introduction to next generation sequencing
11:00 – 11:30 Coffee Break
11:30 – 12:30 Overview of bioinformatics
12:30 – 13:30 Merging the bridge – from data to biological insights
Lunch break
15:00 - 17:30 Hands on to some beginning bioinformatics

Day 2 : RNA-Seq in a nutshell and downstream analysis
09:00 – 11:00 De novo assembly
11:00 – 11:30 Coffee Break
11:30 – 13:30 Read-mapping, RNA-Seq and downstream analysis
15:00 – 17:30 RNA-Seq practical

Day 3 : Applied RNA-Seq and bioinformatics in aquaculture and marine biology – Discussion and feedback
09:00 – 12:00 Applied bioinformatics in crustaceans
12:00 – 12:30 Coffee Break
12:30 – 13:30 Pitfalls in RNA-Seq, - Feedback, Q&A
15:00 – 17:30 RNA-Seq practical – continued

Number of hours: 

12T + 6P


9:00 to 13:30 (coffee break at 11) and 15:00 to 17:30 (coffee break at 16)


Institut de Ciències del Mar

Maximum number of places: 


Special requirements: 

Bring your own laptop and please make sure you have administrative rights to install programs.

Contact for further information: 

For additional information, please check our Contact section or get in contact with the course coordinator maria.pastor "at"


Full price of this course is 290€. A reduced registration fee of 230€ is applied for early registrations (until June 6).
The course fee for ICM/CSIC personnel is 108€.

Price: 290.00€

Cancelation policies

Cancellations by attendants

To cancel a place that you have booked for a Barcelona Ocean course, a notice of cancellation must be sent via email to The following policies apply:
• If you cancel at least 3 months before the start of the course you will receive a full refund.
• If you cancel between 3 months and 1 month prior to the start of the course you will receive a full refund minus a 10% administration fee.
• If you cancel between 1 month and 10 working days prior to the start of the course you will receive a 50% refund.
• If you cancel less than 10 working days before the event you will not be entitled to a refund.
There will be no refund for non-attendance to the course.

Cancellations by Barcelona Ocean

Barcelona Ocean courses are constantly updated and, while we endeavour to deliver the courses as advertised, there may inevitably be occasions when we will have to change some of the course contents or one or more of the lecturers without prior notice or, in very exceptional circumstances, to cancel an event.
In the case of a complete course cancellation, attendants will either be offered an alternative date, a credit note or a full refund. Barcelona Ocean will not compensate for travel fees or any other expense incurred by you as a result of such cancellation. Every effort will be made to avoid the cancellation of any planned courses.

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